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Lonare, M. K.

Safety Assessment of Lincomycin Following Repeated Intramuscular Administration in Goats

Abstract Views :361 | PDF Views:0

Authors

S. Singla ¹, V. K. Dumka ¹, Memansha Sharma ¹, M. K. Lonare ¹, S. K. Sharma ¹

Affiliations
1 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, GADVASU, Ludhiana-141004, IN

Source

Toxicology International (Formerly Indian Journal of Toxicology), Vol 22, No 3 (2015), Pagination: 92-95

Abstract

A safety evaluation of lincomycin was carried out to assess clinical impact of intramuscular administration of lincomycin on various haematological and biochemical parameters in goats to establish safety profile of lincomycin. Six healthy female goats were treated with single intramuscular dose of lincomycin @10.0 mg/kg body weight. The blood samples were collected at day 0 (before administration of drugs), and on 6th day of drug administration. The hematological and plasma biochemical analysis were done. No significant alterations (p < 0.05) were found in the mean value of haematological and biochemical parameters during treatment period except for significant elevation in values of Lactate dehydrogenase and Creatinine. Repeated intramuscular administration of lincomycin (10.0 mg/kg) for 5 days in goat was found safe. Thus, lincomycin may be useful to treat bacterial diseases accompanied by fever, pain and other inflammatory condition in goats.

Keywords

Goats, Lincomycin, Safety Assessment.

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References

Collignon P, Courvalin P, Aidara-Kane A. Clinical importance of antimicrobial drugs in human health. Guide to Antimicrobial Use in Animals. UK: Blackwell Publishing. 2008. p. 44–58.

Giguere S. Lincosamides, pleuromutilins, and Streptogramins. Giguere, editor. Antimicrobial Therapy in Veterinary Medicine. 4th ed; 2006.

Barragry TB. Veterinary drug therapy. Baltimore: Lea and Febiger; 1994. p. 251–62.

Papich MG, Riviere JE. Chloramphenicol and derivatives, macrolides, lincosamides, and miscellaneous antimicrobials. Riviere JE, Papich MG, editors. Veterinary Pharmacology and Therapeutics. 9th ed; 2009. p. 963–5.

Bhavsar SK, Verma MP, Thaker AM. Pharmacokinetics, tissue concentration, and safety of multiple dose intravenous administration of ciprofloxacin in cow calves. J Vet Pharmacol Toxicol. 2004; 3(1):27–34.

Khargharia S, Barua CC, Nath N, Bhattachrya M. Blood biochemical studies of enrofloxacin in Yak after intravenous administration. Iranian J Pharmacol Therap. 2007; 6:137–8.

Patel JH, Varia RD, Patel UD, Vihol PD, Bhavsar SK, Thaker AM. Safety of levofloxacin following repeated oral administration in white leg horn layer birds. Vet World. 2009; 2(4):137–9.

More T. Animal clinical biochemistry. New Delhi: Kalyani Publishers; 2006.

Brar RS, Sandhu HS, Singh A. Veterinary clinical diagnosis by laboratory methods. Ludhiana-New Delhi: Kalyani Publishers; 2000.

Hemato-Biochemical Alterations Mediated by Carbendazim Exposure and Protective Effect of Quercetin in Male Rats

Abstract Views :247 | PDF Views:0

Authors

N. V. Patil ¹, M. K. Lonare ¹, M. Sharma ², P. C. Lalhriatpuia ¹, S. P. S. Saini ¹, S. Rampal ¹

Affiliations
1 Department of Veterinary Pharmacology and Toxicology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana − 141004, Punjab, IN
2 Department of Veterinary Physiology and Biochemistry, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana − 141004, Punjab, IN

Source

Toxicology International (Formerly Indian Journal of Toxicology), Vol 25, No 1 (2018), Pagination: 7-18

Abstract

The main objective of study was to investigate the effect of Carbendazim (CBZ) a most popular agriculture fungicide on hemato-biochemical alterations in male rats and possible ameliorative effect of quercetin. Sprague Dawley male rats were administered CBZ @100, 200 and 400 mg/kg body weight, PO and quercetin (25mg/kg body weight, PO) alone and concomitant in corn oil for the period of 28 days. At the end of exposure period, blood was collected and hemato-biochemical parameters were evaluated in blood and plasma samples. Sub-acute exposure of carbendazim resulted in non-significant decrease (p > 0.05) in haemoglobin, PVC and TEC and increase in MCV, MCH and platelets count in CBZ treated groups. Moreover, administration of CBZ significantly increased (p < 0.05) the plasma ALT, AST, ALP, cholesterol and triglyceride while, significantly decreased (p < 0.05) the plasma protein and globulin level was noticed. Non-significant change in calcium, chloride and phosphorous was observed in CBZ alone treated groups. However, quercetin supplementation concomitant in CBZ treated animals prevented and restored the alterations caused by exposure of CBZ. This study concluded that quercetin has ability to ameliorate the toxic effect caused by CBZ on hemato-biochemical biomarkers in living beings.

Keywords

Carbendazim, Haemato-Biochemical, Quercetin, Rats.

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References

Costa LG, Giordano G, Guizzetti M, Vitalone A. Neurotoxicity of pesticides: A brief review, Front Biosci. 2008; 13:1240−49. https://doi.org/10.2741/2758. PMid: 17981626.

Larramendy ML, Nikoloff N, de Arcaute C R. Genotoxicity and cytotoxicity exerted by pesticides in different biotic matrices: An overview of more than a decade of experimental evaluation, J Environ Toxicol. 2015; 4:225. https://doi.org/10.1201/b18616-3.

Li S, Cao C, Shi H, Yang S, Qi L, Zhao X, Sun C. Effect of quercetin against mixture of four organophosphate pesticides induced nephrotoxicity in rats, Xenobiotica. 2016; 46:225−33. https://doi.org/10.3109/00498254.2015.1070443. PMid: 26226520.

Maria-Lourdes A, Mineko S, Margarita C, Angelica S, Maria-Isabe S, Victor T, Fabiola-Gabriela Z, Ana-Rosa R. Hepatic effects from subacute exposure to insecticides in adult male wistar rats. In: Perveen F, editor. InsecticidesAdvances In Integrated Pest Management Insecticides. Karnal, India: Intech Press; 2012. p. 280−90.

Uggini GK, Patel PV, Balakrishnan S. 2012. Embryotoxic and teratogenic effects of pesticides in chick embryos: a comparative study using two commercial formulations, Environ Toxicol. 2012; 27:166−74. https://doi.org/10.1002/tox.20627.

Selmanoglu G, Barlas N, Songur S, Kocskaya EA. Carbendazim-induced haematological, biochemical and histopathological changes to the liver and kidney of male rats, Hum Exp Toxicol. 2001; 20:625−30. https://doi.org/10.1191/096032701718890603. PMid: 11936576.

Hsu YH, Chang CW, Chen MC, Yuan CY. Carbendaziminduced androgen receptor expression antagonized by flutamide in male rats, J Food Drug Anal. 2011; 19:418−28.

Waghe P, Saini SPS, Rampal S, Prakash N, Lokesh LV. Subchronic exposure to carbendazim induces biochemical and hematological alterations in male goats, Toxicol Environ Chem. 2013; 95:330−36. https://doi.org/10.1080/02772248 .2013.770859.

Ranjan B, Daundkar PS, Rampal S. Ameliorative effect of selenium on carbendazim induced oral sub-chronic testicular toxicity in bucks, Small Rumin Res. 2014; 119:107−13. https://doi.org/10.1016/j.smallrumres.2014.03.006.

Sakr SA, Shalaby SY. Carbendazim-induced testicular damage and oxidative stress in albino rats: ameliorative effect of licorice aqueous extract, Toxicol Ind Health. 2014; 30:259−67. https://doi.org/10.1177/0748233712456059. PMid: 22903170.

Cedric P, Sebastien V, Karim A, Philippe D, Marie-Helene P, Marie-Roberte G, Philippe B, Odette P. Ex vivo assessment of testicular toxicity induced by carbendazim and iprodione, alone or in a mixture, ALTEX. 2016; 33:(4).

Salihu M, Ajayi BO, Adedara IA Farombi EO. 6-Gingerolrich fraction from Zingiber officinale prevents hematotoxicity and oxidative damage in kidney and liver of rats exposed to carbendazim, J Diet Suppl. 2016; 13:433−48. https://doi.org/10.3109/19390211.2015.1107802. PMid: 26673969.

Hashem MA, Mohamed WA, Attia ES. Assessment of protective potential of Nigella sativa oil against carbendazim-and/ or mancozeb-induced hematotoxicity, hepatotoxicity, and genotoxicity, Environ Sci Pollut Res. 2017; 1−13.

Daundkar PS, Rampal S. Evaluation of ameliorative potential of selenium on carbendazim induced oxidative stress in male goats, Environ Toxicol Pharmacol. 2014; 38(3):711−19. https://doi.org/10.1016/j.etap.2014.09.007. PMid: 25299847.

Ahmadi N, Mandegary A, Jamshidzadeh A, MohammadiSardoo M, Mohammadi-Sardo M, Salari E, et al. Hematological abnormality, oxidative stress, and genotoxicity induction in the greenhouse pesticide sprayers; investigating the role of NQO1 gene polymorphism, Toxics. 2018; 6:13. https://doi.org/10.3390/toxics6010013. PMid: 29414880, PMCid: PMC5874786.

Agrawal A, Sharma B. Pesticides induced oxidative stress in mammalian systems, Int J Biol Med Res. 2010; 3:90−104.

Mena S, Ortega A, Estrela JM. Oxidative stress in environmental induced carcinogenesis, Mutat Res. 2009; 674:36−44. https://doi.org/10.1016/j.mrgentox.2008.09.017. PMid: 18977455.

Galal MK, Khalaf AA, Ogaly HA, Ibrahim MA. Vitamin E attenuates neurotoxicity induced by deltamethrin in rats, BMC Complement Altern Med. 2014; 14:458. https://doi.org/10.1186/1472-6882-14-458. PMid:25439240, PMCid: PMC4265463.

Adedara IA, Vaithinathan S, Jubendradass R, Mathur PP, Farombi EO. Kolaviron prevents carbendazim-induced steroidogenic dysfunction and apoptosis in testes of rats, Environ Toxicol Pharmacol. 2013; 35(3):444−53. https://doi.org/10.1016/j.etap.2013.01.010. PMid: 23474402.

Moon SK, Cho GO, Jung SY, Gal SW, Kwon TK, Lee YC, Madamanchi NR, Kim CH. Quercetin exerts multiple inhibitory effects on vascular smooth muscle cells: role of ERK1/2, cell-cycle regulation, and matrix metalloproteinase-9, Biochem Biophys Res Commun. 2003; 301(4):1069−78. https://doi.org/10.1016/S0006-291X(03)00091-3.

Pattanashetti LA, Taranalli AD, Parvatrao V, Malabade RH, Kumar D. Evaluation of neuroprotective effect of quercetin with donepezil in scopolamine-induced amnesia in rats, Indian J Pharmacol. 2017; 49(1):60−64. PMid: 28458424, PMCid: PMC5351240.

Unsal C, Kanter M, Aktas C, Erboga M. Role of quercetin in cadmium-induced oxidative stress, neuronal damage, and apoptosis in rats, Toxicol Ind Health. 2015; 31:1106−15. https://doi.org/10.1177/0748233713486960. PMid:23645211.

Pany SU, Pal AB, Sahu PK. Neuroprotective effect of quercetin in neurotoxicity induced rats: role of neuroinflammation in neurodegeneration, Asian J Pharm Clin Res.2014; 7:152−56.

Ola MS, Ahmed MM, Shams S, Al-Rejaie SS. Neuroprotective effects of quercetin in diabetic rat retina, Saudi J Biol Sci. 2016; 24:1186−94 https://doi.org/10.1016/j.sjbs.2016.11.017. PMid: 28855811, PMCid: PMC5562465.

Aly HA, Domenech O, Abdel-Naim AB. Aroclor 1254 impairs spermatogenesis and induces oxidative stress in rat testicular mitochondria, Food Chem Toxicol. 2009; 47(8):1733−38. https://doi.org/10.1016/j.fct.2009.03.019. PMid: 19306909.

Teo S, Stirling D, Thomas S, Hoberman A, Kiorpes A, Khetani V. A 90-day oral gavage toxicity study of d-methylphenidate and d, l-methylphenidate in SpragueDawley rats, Toxicology. 2002; 179(3):183−96. https://doi.org/10.1016/S0300-483X(02)00338-4.

Bhardwaj S, Srivastava M K, Kapoor U, Srivastava L P. A 90 days oral toxicity of imidacloprid in female rats: Morphological, biochemical and histopathological evaluations, Food Chem Toxicol. 2010; 48:1185−90. https://doi.org/10.1016/j.fct.2010.02.009. PMid: 20146932.

Singh TB, Mukhopadhayay SK, Sar TK, Ganguly S. Acetamiprid induces toxicity in mice under experimental conditions with prominent effect on the hematobiochemical parameters, J J Drug Metab Toxicol. 2012; 3(6):134. https://doi.org/10.4172/2157-7609.1000134.

El-Desoky G, Abdelreheem M, Abdulaziz AO, ALOthman Z, Mahmoud M, Yusuf K. Potential hepatoprotective effects of vitamin E and selenium on hepatotoxicity induced by malathion in rats, Afr J Pharm Pharmacol. 2012; 6:806−13. https://doi.org/10.5897/AJPP11.628.

Sharma D, Sangha GK. Effects of glucosinolates rich broccoli extract against triazophos induced toxicity in wistar rats, J Biomed Sci. 2016; 5:25. https://doi.org/10.4172/2254-609X.100039.

Gholami M, Khayat ZK, Anbari K, Obidavi Z, Varzi A, Boroujeni MB, Alipour M, Niapoor A, Gharravi AM. Quercetin ameliorates peripheral nerve ischemia-reperfusion injury through the NF-kappa B pathway, Anat Sci Int. 2017; 92:330−37. https://doi.org/10.1007/s12565-016-0336-z. PMid: 26972295.

Etim NN, Williams ME, Akpabio U, Offiong EE. Haematological parameters and factors affecting their values, Agri Sci. 2014; 2:37−47. https://doi.org/10.12735/as.v2i1p37.

Owoeye O, Adedara IA, Bakare OS, Adeyemo OA, Egun C, Farombi EO. Kolaviron and vitamin E ameliorate hematotoxicity and oxidative stress in brains of prepubertal rats treated with an anticonvulsant phenytoin, Toxicol Mech Methods. 2014; 24:353−61. https://doi.org/10.3109/153765 16.2014.913752. PMid: 24712692.

Murray RK, Granner DK, Mayes PA, Rodwell VW, editors. Harper’s Illustrated Biochemistry, International. New York: The McGraw-Hill Companies; 2007. p. 46−47. PMid: 17486773.

Zari TA, Al-Attar AM. Therapeutic effects of olive leaves extract on rats treated with a sublethal concentration of carbendazim, Eur Rev Med Pharmacol Sci. 2011; 15(4):413−26. PMid: 21608437.

Suradkar SG, Ghodasara DJ, Vihol P, Patel J, Jaiswal V, Prajapati KS. Haemato-biochemical alterations induced by lead acetate toxicity in wistar rats, Vet World. 2009; 2:429−31.

Balani T, Agrawal S, Thaker AM. Hematological and biochemical changes due to short-term oral administration of imidacloprid, Toxicol Int. 2011; 18:2. https://doi.org/10.4103/0971-6580.75843. PMid: 21430911, PMCid: PMC3052578.

Omonona AO, Jarikre TA. Effect of carbendazim exposure and vitamin E supplementation in African Giant rats, J Agri Ecol Res Int. 2015; 4:1−9. https://doi.org/10.9734/JAERI/2015/16715.

Farag A, Ebrahim H, ElMazoudy R, Kadous E.Developmental toxicity of fungicide carbendazim in female mice, Birth Defects Res B Dev Reprod Toxicol. 2011; 92(2):122−30 https://doi.org/10.1002/bdrb.20290. PMid: 21416578.

Kalender Y, Uzunhisarcikli M, Ogutcu A, Acikgoz F, Kalender S. Effects of diazinon on pseudocholinesterase activity and haematological indices in rats: the protective role of vitamin E, Environ Toxicol Pharmacol. 2006; 22:46−51. https://doi.org/10.1016/j.etap.2005.11.007. PMid: 21783685.

Agbor GA, Oben JE, Nkegoum B, Takala JP, Ngogang JY. Hepatoprotective activity of Hibiscus cannabinus (Linn.) against carbon tetrachloride and paracetamol induced liver damage in rats, Pak J Biol Sci. 2005; 8:1397−401. https:// doi.org/10.3923/pjbs.2005.1397.1401.

Roberts TR, Huston DH, Lee PW, Nicholls PH, Plimmer JR, editors. Metabolic pathways of Agrochemicals. Part 2: Insecticides and fungicides. Cambridge: The Royal Society of Chemistry; 1999. https://doi.org/10.1039/9781847551375.

Ganong WF. Review of Medical Physiology. 19th ed. Appleton and Lange, Stamford, Connecticut, USA; 1999. p. 267−80.

Wang Y, Gilbreath III TM, Kukutla P, Yan G, Xu J. Dynamic gut microbiome across life history of the malaria mosquito Anopheles gambiae in Kenya, PLoS One. 2011; 6:e24767. https://doi.org/10.1371/journal.pone.0024767. PMid: 21957459, PMCid: PMC3177825.

Rivera L, Moron R, Sanchez M, Zarzuelo A, Galisteo M. Quercetin ameliorates metabolic syndrome and improves the inflammatory status in obese zucker rats, Obesity. 2008; 16:2081−87. https://doi.org/10.1038/oby.2008.315. PMid: 18551111.

Kobori M, Takahashi Y, Akimoto Y, Sakurai M, Matsunaga I, Nishimuro H, Ippoushi K, Oike H, Ohnishi-Kameyama M. Chronic high intake of quercetin reduces oxidative stress and induces expression of the antioxidant enzymes in the liver and visceral adipose tissues in mice, J Funct Foods. 2015; 15:551−60. https://doi.org/10.1016/j.jff.2015.04.006.

Kandere‐Grzybowska K, Kempuraj D, Cao J, Cetrulo CL, Theoharides TC. Regulation of IL‐1‐induced selective IL-6 release from human mast cells and inhibition by quercetin, Br J Pharmacol. 2006; 148:208−15. https://doi.org/10.1038/sj.bjp.0706695. PMid: 16532021, PMCid: PMC1617055.

Singh A, Holvoet S, Mercenier A. Dietary polyphenols in the prevention and treatment of allergic diseases, Clin Exp Allergy. 2011; 41:1346−59. https://doi.org/10.1111/j.1365-2222.2011.03773.x. PMid: 21623967.

Chirumbolo S. The role of quercetin, flavonols and flavones in modulating inflammatory cell function, Inflamm Allergy Drug Targets. 2010; 9:263−85. https://doi.org/10.2174/187152810793358741. PMid: 20887269.

Finn DF, Walsh JJ. Twenty-first century mast cell stabilizers, Br J Pharmacol. 2013; 170:23−37. https://doi.org/10.1111/bph.12138. PMid: 23441583, PMCid: PMC3764846.

Weng Z, Zhang B, Asadi S, Sismanopoulos N, Butcher A, Fu X, Katsarou-Katsari A, Antoniou C, Theoharides TC. Quercetin is more effective than cromolyn in blocking human mast cell cytokine release and inhibits contact dermatitis and photosensitivity in humans, PLoS One. 2012; 7(3):e33805. https://doi.org/10.1371/journal.pone.0033805. PMid: 22470478, PMCid: PMC3314669.

Lakhanpal P and Rai D K. Quercetin: a versatile flavonoid. Internet J Med Update 2007;2:22-37. https://doi.org/10.4314/ijmu.v2i2.39851.

Jurikova T, Mlcek J, Sochor J, Hegedusova A. Polyphenols and their mechanism of action in allergic immune response, Global J Allergy. 2015; 1:37−39. https://doi.org/10.17352/2455-8141.000008.

Toxicological Investigation of Single Oral Dose Administration of Imidacloprid in Male Wistar Rats

Abstract Views :292 | PDF Views:0

Authors

M. K. Lonare ¹, Manoj Kumar ², A. More ², A. G. Telang ²

Affiliations
1 College of Veterinary Sciences, GADVASU, Ludhiana – 141004, Punjab, IN
2 Division of Veterinary Pharmacology and Toxicology, Indian Veterinary Research Institute, Bareilly– 243122, Uttar Pradesh, IN

Source

Toxicology International (Formerly Indian Journal of Toxicology), Vol 26, No 1&2 (2019), Pagination: 8-14

Abstract

The aim of this study was to evaluate the acute toxic effect of Imidacloprid (IM) on adult wistar rats. Rats were divided into four different groups. Animals from Group I and II were received water and corn oil, respectively, while, Group III and IV were received oral dose of IM @ 150mg/kg and 300mg/kg body weight, respectively. Thereafter, animals were subjected to series of neuro-toxicity related parameters (general behavioral changes, motor activity, pentobarbitone induced sleeping time and measurement of pain response through mechanical stimulation, radiant heat and tail clip method). IM administration in rats produced significant behavioral depression which resulted into decrease in alertness, awareness and grip strength, and there was reduction in the response to sound, touch and pinna reflexes as compared control. Further, IM resulted in significant decrease in spontaneous locomotor activity and forced locomotor activity (rota-rod performance). IM administration significantly increased the pentobarbitone-induced sleeping time without altering the onset of sleeping time. Orally administered IM did not produce any significant analgesic or hyperalgesic effect. The present study suggests that IM has pronounced CNS effect without effecting analgesic activity during acute toxicosis in animals.

Keywords

Analgesic Activity, Imidacloprid, Motor Activity, Sleeping Time.

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References

Meienfisch P, Brandl F, Kobel W, Rindlisbacher A, Senn R. Nicotinoid insecticides and nicotinic acetylcholine receptor, Springer, Tokyo; 1999. p. 177. https://doi.org/10.1007/978-4-431-67933-2_8

Ferrer I, Thurman EM, Fernández-Alba AR. Quantitation and accurate mass analysis of pesticides in vegetables by LC/TOF-MS. Analytical Chemistry. 2005; 77:2818–25. https://doi.org/10.1021/ac048458x. PMid:15859598

Nauen R, Ebbinghaus-Kintscher U, Schmuck R. Toxicity and nicotinic acetylcholine receptor interaction of imidacloprid and its metabolites in Apis mellifera (Hymenoptera: Apidae). Pest Management Science. 2001; 57:577–86. https://doi.org/10.1002/ps.331. PMid:11464788

Muccio Di A, Fidente P, Barbini DA, Dommarco R, Seccia S, Morrica P. Application of solid-phase extraction and liquid chromatography-mass spectrometry to the determination of neonicotinoid pesticide residues in fruit and vegetables. Journal of Chromatography-A. 2006; 1108:1-6. https://doi.org/10.1016/j.chroma.2005.12.111. PMid:16448655

Butcherine P, Benkendorff K, Kelaher B, Barkla BJ. The risk of neonicotinoid exposure to shrimp aquaculture. Chemosphere. 2018; 217:329–48. https://doi.org/10.1016/j.chemosphere.2018.10.197. PMid:30419387.

Song MY, Brown JJ. Osmotic effects as a factor modifying insecticide toxicity on Aedes and Ecotoxicology and Environmental Safety. 1998; 41:195–202. https://doi.org/10.1006/eesa.1998.1693. PMid:9756708

Hovda LR, Hooser SB. Toxicology of newer pesticides for use in dogs and cats. Veterinary Clinics: Small Animal Practice. 2002; 32: 455–67. https://doi.org/10.1016/S0195-5616(01)00013-4

Matsuda KM, Shimomura Y, Kondo M, Ihara K, Hashigami N, Yoshida V, et al. Role of loop D of the α7-nicotinic acetylcholine receptor in its interaction with the insecticide imidacloprid and related neonicotinoids. British Journal of Pharmacology. 2000; 130:981–6. https://doi.org/10.1038/sj.bjp.0703374. PMid:10882381. PMCid:PMC1572150

Qadir S, Iqbal F. Effect of subleathal concentrtion of imidacloprid on the histology of heart, liver and kidney in Labeo rohitr. Pakistan Journal of Pharmaceutical Sciences. 2016; 29(6):2033–8.

Burke AP, Niibori Y, Terayama H, Ito M, Pidgeon C, Arsenault J, et al. Mammalian susceptibility to a neonicotinoid insecticide after fetal and early postnatal exposure. Scientific Reports. 2018; 8(1):16639. https:// doi.org/10.1038/s41598-018-35129-5. PMid:30413779. PMCid:PMC6226530

Guo J, Shi R, Cao Y, Luan Y, Zhou Y, Gao Y, et al. Genotoxic effects of imidacloprid in human lymphoblastoid TK6 cells. Drug and Chemical Toxicology. 2018; 13:1–5. https://doi.org/10.1080/01480545.2018.1497048. PMid:30103639

Iturburu FG, Simoniello MF, Medici S, Panzeri AM, Menone ML. Imidacloprid causes DNA damage in fish: Clastogenesis as a mechanism of genotoxicity. Bulletin of Environmental Contamination and Toxicology. 2018; 100(6):760–4. https://doi.org/10.1007/s00128-018-2338-0. PMid:29663041

Pike KS, Reed GL, Graf GT, Allison D. Compatibility of imidacloprid with fungicides as a seed-treatment control of Russian wheat aphid (Homoptera: Aphidae) and effect on germination, growth and Yield of Wheat Barley. Journal of Economic Entomology. 1993; 86:586–93. https://doi.org/10.1093/jee/86.2.586

Scholz K, Spiteller M. Influence of groundcover on the degradation of 14C-imidacloprid in soil. Proceeding of the Brighton Crop Protection Conference, UK, Germany; 1992.

Duzguner V, Erdogan S. Acute oxidant and inflammatory effects of imidacloprid on the mammalian central nervous system and liver in rats. Pesticide Biochemistry and Physiology. 2010; 97:13–18. https://doi.org/10.1016/j.pestbp.2009.11.008

Vesile D, Suat E. Acute oxidant and inflammatory effects of imidacloprid on the mammalian central nervous system and liver in rats. Pesticide Biochemistry and Physiology. 2010; 97:13–18. https://doi.org/10.1016/j.pestbp.2009.11.008

Lonare M, Kumar M, Raut S, Badgujar P, Doltade S, Telang A. Evaluation of imidacloprid-induced neurotoxicity in male rats: A protective effect of curcumin. Neurochemistry International. 2014; 78:122–9. https://doi.org/10.1016/j.neuint.2014.09.004. PMid:25261201

Bhardwaj S, Srivastava MK, Kapoor U, Srivastava LP. A 90 days oral toxicity of imidacloprid in female rats: Morphological, biochemical and histopathological evaluations. Food and Chemical Toxicology. 2010; 48:1185–90. https://doi.org/10.1016/j.fct.2010.02.009. PMid:20146932

Najafi G, Razi M, Hoshyar A, Shahmohamadloo S, Feyzi S. The effect of chronic exposure with imidacloprid insecticide on fertility in mature male rats. International Journal of Fertility & Sterility. 2010; 4:9–16.

Lonare M, Kumar M, Raut S, More A, Doltade S, et al. Evaluation of ameliorative effect of curcumin on imidacloprid-induced male reproductive toxicity in wistar rats. Environmental Toxicology. 2016; 31(10):1250–63. https:// doi.org/10.1002/tox.22132. PMid:25758541

OECD. Organization for economic cooperation and development. OECD Guidelines for Testing of Chemicals. Guideline 423, Acute Oral Toxicity-Acute Toxic Class Method, Adopted, March 22; 1996.

Dunham NW, Miya TS. A note on simple apparatus for detecting neurological deficit in rats and mice. American Journal of Pharmacology. 1957; 46:208–9. https://doi.org/10.1002/jps.3030460322. PMid:13502156

Randall LO, Selitto JJ. A method for measurement of analgesic activity of inflammed tissue. Archives Internationales de Pharmacodynamie et de Therapie. 1957; 3:409–19.

Takagi H, Inukai T, Nakam M. Modification of Haffiners method for testing analgesics. The Japanese Journal of Pharmacology. 1966; 16:287–95. https://doi.org/10.1254/jjp.16.287. PMid:5298309

Dandiya PC, Collumbine H. Studies on Acorus calamus (L.) some pharmacological action of the volatile oil. Journal of Pharmacology and Experimental Therapeutics. 1956; 125:353–9.

Avery ML, Decker DG, Fischer DL. Cage and flight pen evaluation of avian repellancy and hazard associated with imidacloprid-treated rice seed. Crop Protection. 1994; 13:535–40. https://doi.org/10.1016/0261-2194(94)90107-4

Yeh IJ, Lin TJ, Hwang DY. Acute multiple organ failure with imidacloprid and alcohol ingestion. The American Journal of Emergency Medicine. 2010; 28:255. https://doi.org/10.1016/j.ajem.2009.05.006 . PMid:20159407

Chemical Watch Fact Sheet. Beyond Pesticides 701 E Street SE #200 Washington, DC 2003; 202:543–5.

Tomizawa M, Casida JE. Minor structural changes in nicotinoid insecticides confer differential subtype selectivity for mammalian nicotinic acetylcholine receptors. British Journal of Pharmacology. 1999; 127:115–22. https://doi.org/10.1038/sj.bjp.0702526. PMid:10369463. PMCid:PMC1566001

Tomizawa M, Casida JE. Desnitro-imidacloprid activates the extracellular signal-regulated kinase cascade via the nicotinic receptor and intracellular calcium mobilization in N1E-115 cells. Toxicology and Applied Pharmacology. 2002; 184:180–6. https://doi.org/10.1006/taap.2002.9503. PMid:12460746

Manna S, Bhattacharyya D, Mandal TK, Day S. Neuropharmacological effect of alfa-cypermethrin in rats. Indian J Pharmacol. 2005; 37:18–20. https://doi.org/10.4103/0253-7613.13849

Tomizawa M, Cowan A, Casida JE. Analgesic and toxic effects of neonicotinoid insecticides in mice. Toxicology and Applied Pharmacology. 2001; 177:77–83. https://doi.org/10.1006/taap.2001.9292. PMid:11708903

Tripathi HL, Martin BR, Aceto MD. Nicotine-induced antinociception in rats and mice: Correlation with nicotine brain levels. Journal of Pharmacology and Experimental Therapeutics. 1982; 221:91–6.

Toxicological Sequelae of Pesticide Combinations Exposure in Buffalo Mesenchymal Stem Cells under In Vitro

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Authors

H. Singh ¹, M. K. Lonare ¹, M. Sharma ², R. Udheya ³, S. Singla ¹, V. K. Dumka ¹

Affiliations
1 Department of Veterinary Pharmacology and Toxicology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141004, Punjab, IN
2 Department of Veterinary Physiology and Biochemistry, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141004, Punjab, IN
3 Department of Veterinary Surgery and Radiology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141004, Punjab, IN

Source

Toxicology International (Formerly Indian Journal of Toxicology), Vol 29, No 1 (2022), Pagination: 1-14

Abstract

The presence of one or more pesticides in a variety of mediums is responsible for their indirect toxicological events leading to cell senescence. In the present investigation, the endeavor was made to see the effect of pesticides Car-Benda-Zim (CBZ) and IMIdacloprid (IMI) alone and in combination with bone marrow-derived Mesenchymal Stem Cells (bMSCs) of buffalo origin. Isolated and cultured bMSCs were exposed to CBZ and IMI alone and in combinations at lower doses. Cells were observed for alterations in cell morphology, oxidative stress, mitochondrial damage and cellular senescence. bMSCs characterized for stem cell surface markers and found to be positive for AP, CD73 and OCT4. bMSCs exposed to IC25, IC12.5 and IC6.25 CBZ and IMI alone and combinations of IC12.5 and IC6.25 of CBZ and IMI. Results revealed significant reduction (p≤0.05) in cell viability noticed on microscopic examination along with loss of normal cell morphology and increased in Reactive Oxygen Species (ROS) positive cells, cells with loss of ΔΨm and number of senescent cells in CBZ and IMI treated groups. Lower dose combination groups showed elevated effects when compared with higher dose alone treated groups and control groups. Present findings suggest that CBZ and IMI induced cytotoxicity in bMSCs mediated via ROS production, altered ΔΨm leading to the cell damage and predisposing senescence process. Moreover, the co-existence of CBZ and IMI in a medium has a considerably more toxic effect than their individual effect.

Keywords

Carbendazim, Imidacloprid, Stem Cells, Mitochondrial Transmemberane Potential, Reactive (ROS), Senescence

Full Text

References

Damalas CA, Koutroubas SD. Farmers’ Training on pesticide use is associated with elevated safety behavior. Toxics. 2017; 5(3):19. https://doi.org/10.3390/toxics5030019. PMID: 29051451; PMCID: PMC5634698.

Patil N, Lonare M, Sharma M, Lalhriatpuia P, Saini S, Rampal S. Hemato-biochemical alterations mediated by carbendazim exposure and protective effect of quercetin in male rats. Toxicol Int. 2018; 25(1):1-12. https://doi:10.22506/ti/2018/v25/i1/21569.

Çevik UA, Sağlık BN, Korkut B, Özkay Y, Ilgın S. Antiproliferative, cytotoxic, and apoptotic effects of new benzimidazole derivatives bearing hydrazone moiety. J Heterocyclic Chemistry. 2017; 55:138. https://doi:10.1002/jhet.3016.

Singh H, Lonare MK, Sharma M, Udehiya R, Singla S, Saini SP, Dumka VK. Interactive effect of carbendazim and imidacloprid on buffalo bone marrow derived mesenchymal stem cells: Oxidative stress, cytotoxicity and genotoxicity. Drug Chem Toxicol. 2021; 29:1-15. https://doi.org/10.1080/01480545.2021.2007023. PMID: 34844488.

Caron-Beaudoin E, Viau R, Hudon-Thibeault AA, Vaillancourt C, Sanderson JT. The use of a unique co-culture model of fetoplacental steroidogenesis as a screening tool for endocrine disruptors: The effects of neonicotinoids on aromatase activity and hormone production. Toxicol Appl Pharmacol. 2017; 332:15-24. https://doi.org/10.1016/j.taap.2017.07.018. PMID: 28750898.

Lonare M, Kumar M, Raut S, Badgujar P, Doltade S, Telang A. Evaluation of imidacloprid-induced neurotoxicity in male rats: A protective effect of curcumin. Neurochem Int. 2014; 78:122-129. https://doi.org/10.1016/j.neuint.2014.09.004. PMID: 25261201.

Chakroun, Intissar G, Lobna E, Oumaïma A, Fadoua N, Emna K, Najjar MF, Zohra H, Hassen BC. Imidacloprid enhances liver damage in Wistar rats: Biochemical, oxidative damage and histological Assessment. J Coastal Life Med. 2017; 5(12):540-546. https://doi.org/10.12980/jclm.5.2017J7-149.

Casida JE. Pesticide interactions: Mechanisms, benefits, and risks. J Agric Food Chem. 2017; 65(23):4553-4561. https://doi:10.1021/acs.jafc.7b01813.

Hernández AF, Gil F, Lacasaña M. Toxicological interactions of pesticide mixtures: An update. Arch Toxicol. 2017; 91(10):3211-3223. https://doi.org/10.1007/s00204-017-2043-5. PMID: 28845507.

Lonare MK, Vemu B, Singh AK, Dumka VK, Singla S, Sharma SK. Cytotoxicity and oxidative stress alterations induced by aldrin in BALB/c 3T3 fibroblast cells. Proc Natl Acad Sci India Sect B Biol Sci. 2017; 87:1209-1216. https://doi.org/10.1007/s40011-015-0694-7.

Gade NE, Pratheesh MD, Nath A, Dubey PK, Amarpal, Sharma B, Saikumar G, Taru Sharma G. Molecular and cellular characterization of buffalo bone marrow-derived mesenchymal stem cells. Reprod Domest Anim. 2013; 48(3):358-367. https://doi.org/10.1111/j.1439-0531.2012.02156.x.

Devi P, Sharma M, Singh SD, Lonare MK, Udehiya R. Viability and expression pattern of cryopreserved mesenchymal stem cells derived from buffalo bone marrow. Ruminant Sci. 2017; 6(1):7-12.

Aranda A, Sequedo L, Tolosa L, Quintas G, Burello E, Castell JV, Gombau L. Dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay: a quantitative method for oxidative stress assessment of nanoparticle-treated cells. Toxicol In Vitro. 2013; 27(2):954-963. https://doi.org/10.1016/j.tiv.2013.01.016. PMID: 23357416.

Salmon ED, Shaw SL, Waters JC, Waterman-Storer CM, Maddox PS, Yeh E, Bloom K. A high-resolution multimode digital microscope system. Methods Cell Biol. 2007; 81:187-218. https://doi.org/10.1016/S0091-679X(06)81011-3. PMID: 17519169.

Doan CC, Truong NH, Vu NB, Nguyen TT, Nguyen HM, Nguyen KG, Do S, Phan NK, Pham PV. Isolation, culture and cryopreservation of human bone marrow derived mesenchymal stem cells. Int J Plant Animal Env Sci. 2012; 2(2):358-367.

Yu Y, Yao AH, Chen N, Pu LY, Fan Y, Lv L, Sun BC, Li GQ, Wang XH. Mesenchymal stem cells over-expressing hepatocyte growth factor improve small-for-size liver grafts regeneration. Mol Ther. 2007; 15(7):1382-1389. https://doi.org/10.1038/sj.mt.6300202. PMID: 17519892.

de Macedo Braga LM, Lacchini S, Schaan BD, Rodrigues B, Rosa K, De Angelis K, Borges LF, Irigoyen MC, Nardi NB. In situ delivery of bone marrow cells and mesenchymal stem cells improves cardiovascular function in hypertensive rats submitted to myocardial infarction. J Biomed Sci. 2008; 15(3):365-374. https://doi.org/10.1007/s11373-008-9237-z. PMID: 18256904.

Peterbauer-Scherb A, van Griensven M, Meinl A, Gabriel C, Redl H, Wolbank S. Isolation of pig bone marrow mesenchymal stem cells suitable for one-step procedures in chondrogenic regeneration. J Tissue Eng Regen Med. 2010; 4(6):485-490. https://doi.org/10.1002/term.262. PMID: 20112279.

McCarty RC, Gronthos S, Zannettino AC, Foster BK, Xian CJ. Characterisation and developmental potential of ovine bone marrow derived mesenchymal stem cells. J Cell Physiol. 2009; 219(2):324-333. https://doi.org/10.1002/jcp.21670. PMID: 19115243.

Rentsch C, Hess R, Rentsch B, Hofmann A, Manthey S, Scharnweber D, Biewener A, Zwipp H. Ovine bone marrow mesenchymal stem cells: isolation and characterization of the cells and their osteogenic differentiation potential on embroidered and surface-modified polycaprolactone-co-lactide scaffolds. In Vitro Cell Dev Biol Anim. 2010; 46(7):624-634. https://doi.org/10.1007/s11626-010-9316-0. PMID: 20490706.

Honda H, Tomizawa M, Casida JE. Neo-nicotinoid metabolic activation and inactivation established with coupled nicotinic receptor-CYP3A4 and -aldehyde oxidase systems. Toxicol Lett. 2006; 161(2):108-114. https://doi.org/10.1016/j.toxlet.2005.08.004. PMID: 16153789.

Tomizawa M, Lee DL, Casida JE. Neonicotinoid insecticides: molecular features conferring selectivity for insect versus mammalian nicotinic receptors. J Agric Food Chem. 2000; 48(12):6016-6024. https://doi.org/10.1021/jf000873c. PMID: 11312774.

Abolaji AO, Awogbindin IO, Adedara IA, Farombi EO. Insecticide chlorpyrifos and fungicide carbendazim, common food contaminants mixture, induce hepatic, renal, and splenic oxidative damage in female rats. Hum Exp Toxicol. 2017; 36(5):483-493. https://doi.org/10.1177/0960327116652459. PMID: 27268782.

Chauhan LK, Varshney M, Pandey V, Sharma P, Verma VK, Kumar P, Goel SK. ROS-dependent genotoxicity, cell cycle perturbations and apoptosis in mouse bone marrow cells exposed to formulated mixture of cypermethrin and chlorpyrifos. Mutagenesis. 2016; 31(6):635-642. https://doi.org/10.1093/mutage/gew031. PMID: 27470700.

Abhishek A, Ansari NG, Shankhwar SN, Jain A, Singh V. In vitro toxicity evaluation of low doses of pesticides in individual and mixed condition on human keratinocyte cell line. Bioinformation. 2014; 31;10(12):716-720. https://doi.org/10.6026/97320630010716. PMID: 25670872.

Sands M, Kron MA, Brown RB. Pentamidine: a review. Rev Infect Dis. 1985; 7(5):625-634. https://doi.org/10.1093/clinids/ 7.5.625. PMID: 3903942.

Vercesi AE, Docampo R. Ca2+ transport by digitonin-permeabilized Leishmania donovani. Effects of Ca2+, pentamidine and WR-6026 on mitochondrial membrane potential in situ. Biochem J. 1992; 284 (Pt 2)(Pt 2):463-467. https://doi.org/10.1042/bj2840463. PMID: 1376113.

Andréo R, Regasini LO, Petrônio MS, Chiari-Andréo BG, Tansini A, Silva DH, Cicarelli RM. Toxicity and Loss of Mitochondrial Membrane Potential Induced by Alkyl Gallates in Trypanosoma cruzi. Int Sch Res Notices. 2015; 2015:924670. https://doi:10.1155/2015/924670. PMID: 27347554.

Sakamuru S, Attene-Ramos MS, Xia M. Mitochondrial Membrane Potential Assay. Methods Mol Biol. 2016; 1473:17-22. https://doi.org/10.1007/978-1-4939-6346-1_2. PMID: 27518619.

Area-Gomez E, Del Carmen Lara Castillo M, Tambini MD, Guardia-Laguarta C, de Groof AJ, Madra M, Ikenouchi J, Umeda M, Bird TD, Sturley SL, Schon EA. Upregulated function of mitochondria-associated ER membranes in Alzheimer disease. EMBO J. 2012; 5; 31(21):4106-4123. https://doi.org/10.1038/emboj.2012.202. PMID: 22892566.

Adachi M, Ishii H. Role of mitochondria in alcoholic liver injury. Free Radic Biol Med. 2002; 32(6):487-491. https://doi.org/10.1016/s0891-5849(02)00740-2. PMID: 11958949.

Campisi J. Aging, cellular senescence, and cancer. Annu Rev Physiol. 2013; 75:685-705. https://doi.org/10.1146/annurev-physiol-030212-183653. PMID: 23140366.

Shahini A, Choudhury D, Asmani M, Zhao R, Lei P, Andreadis ST. NANOG restores the impaired myogenic differentiation potential of skeletal myoblasts after multiple population doublings. Stem Cell Res. 2018; 26:55-66. https://doi.org/10.1016/j.scr.2017.11.018. PMID: 29245050.

Baker DJ, Childs BG, Durik M, Wijers ME, Sieben CJ, Zhong J, Saltness RA, Jeganathan KB, Verzosa GC, Pezeshki A, Khazaie K, Miller JD, van Deursen JM. Naturally occurring p16(Ink4a)-positive cells shorten healthy lifespan. Nature. 2016; 530(7589):184-189. https://doi.org/10.1038/nature16932. PMID: 26840489.

Bernstein H, Payne CM, Bernstein C, Garewal H, Dvorak K. Cancer and Aging as Consequences of Un-Repaired DNA Damage. New Research on DNA Damages. New York, USA: Nova Science Publishers; 2008. p.1-47.

Pan MR, Li K, Lin SY, Hung WC. Connecting the dots: From DNA damage and repair to Aging. Int J Mol Sci. 2016; 17(5):685. https://doi.org/10.3390/ijms17050685. PMID: 27164092.

Menon R, Boldogh I, Urrabaz-Garza R, Polettini J, Syed TA, Saade GR, Papaconstantinou J, Taylor RN. Senescence of primary amniotic cells via oxidative DNA damage. PLoS One. 2013; 8(12):e83416. https://doi.org/10.1371/journal.pone.0083416. PMID: 24386195.

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